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Usage: Paste a DNA sequence, ID or AC (ex. U73445) and the two primers (ex. forward AAA[AT]AA and reverse AAAGGG), select the format, and the options and click the "Run iPCR" button.
The primer syntax allows the square parenthesis ([]) to specify an alternative and the dot (.) to replace the N for any nucleotide. The degenerated code is not allowed.
Make sure that the format button shows the correct format.
Select format:

Query title (option)
Paste your sequence here:
(or ID or accession number)
Paste Forward primer: (5'->3')
Paste Reverse primer: (5'->3')
minimal amplified length (bp)
maximal amplified length (bp)